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reference identifiers additional information antibody dnmt1  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology reference identifiers additional information antibody dnmt1
    Figure 1. DNA hypomethylation results in the activation of L1TD1 expression and loss of L1TD1 affects cell viability in HAP1 cells. (A) Quantification of DNA methylation levels at the L1TD1 promoter in HAP1 wildtype (WT), <t>DNMT1</t> KO, and DNMT1/L1TD1 DKO cells using the MethyLight assay. DNA methylation is shown as percentage of methylation ratio (PMR). (B) qRT-PCR analysis of L1TD1 mRNA expression in HAP1 WT, DNMT1 KO, and DNMT1/ L1TD1 DKO cells. GAPDH was used as a normalization control and relative L1TD1 mRNA levels in DNMT1 KO cells were set to 1. Data are shown as a
    Reference Identifiers Additional Information Antibody Dnmt1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 575 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+identifiers+additional+information+antibody+dnmt1/10__7554_slash_elife__96850-283-13-23?v=Santa+Cruz+Biotechnology
    Average 95 stars, based on 575 article reviews
    reference identifiers additional information antibody dnmt1 - by Bioz Stars, 2026-07
    95/100 stars

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    1) Product Images from "The domesticated transposon protein L1TD1 associates with its ancestor L1 ORF1p to promote LINE-1 retrotransposition"

    Article Title: The domesticated transposon protein L1TD1 associates with its ancestor L1 ORF1p to promote LINE-1 retrotransposition

    Journal: eLife

    doi: 10.7554/elife.96850

    Figure 1. DNA hypomethylation results in the activation of L1TD1 expression and loss of L1TD1 affects cell viability in HAP1 cells. (A) Quantification of DNA methylation levels at the L1TD1 promoter in HAP1 wildtype (WT), DNMT1 KO, and DNMT1/L1TD1 DKO cells using the MethyLight assay. DNA methylation is shown as percentage of methylation ratio (PMR). (B) qRT-PCR analysis of L1TD1 mRNA expression in HAP1 WT, DNMT1 KO, and DNMT1/ L1TD1 DKO cells. GAPDH was used as a normalization control and relative L1TD1 mRNA levels in DNMT1 KO cells were set to 1. Data are shown as a
    Figure Legend Snippet: Figure 1. DNA hypomethylation results in the activation of L1TD1 expression and loss of L1TD1 affects cell viability in HAP1 cells. (A) Quantification of DNA methylation levels at the L1TD1 promoter in HAP1 wildtype (WT), DNMT1 KO, and DNMT1/L1TD1 DKO cells using the MethyLight assay. DNA methylation is shown as percentage of methylation ratio (PMR). (B) qRT-PCR analysis of L1TD1 mRNA expression in HAP1 WT, DNMT1 KO, and DNMT1/ L1TD1 DKO cells. GAPDH was used as a normalization control and relative L1TD1 mRNA levels in DNMT1 KO cells were set to 1. Data are shown as a

    Techniques Used: Activation Assay, Expressing, DNA Methylation Assay, Methylation, Quantitative RT-PCR, Control

    Figure 3. L1TD1 cross-talk with its ancestor L1 ORF1p. (A) Volcano plot displaying the comparison of the proteomes of HAP1 DNMT1 KO and DNMT1/ L1TD1 DKO cells determined by mass spectrometry. Differentially abundant proteins were plotted as DNMT1/L1TD1 DKO over DNMT1 KO (log2FC ≥1, adj. p-value<0.05 [red] and log2FC ≤ –1, adj. p-value<0.05 [blue]). (B) Volcano plot illustrating the DESeq2 analysis of RNA-seq performed with HAP1 DNMT1 KO and DNMT1/L1TD1 DKO cells. Differentially expressed genes are plotted as DNMT1/L1TD1 DKO over DNMT1 KO (log2FC ≥1,
    Figure Legend Snippet: Figure 3. L1TD1 cross-talk with its ancestor L1 ORF1p. (A) Volcano plot displaying the comparison of the proteomes of HAP1 DNMT1 KO and DNMT1/ L1TD1 DKO cells determined by mass spectrometry. Differentially abundant proteins were plotted as DNMT1/L1TD1 DKO over DNMT1 KO (log2FC ≥1, adj. p-value<0.05 [red] and log2FC ≤ –1, adj. p-value<0.05 [blue]). (B) Volcano plot illustrating the DESeq2 analysis of RNA-seq performed with HAP1 DNMT1 KO and DNMT1/L1TD1 DKO cells. Differentially expressed genes are plotted as DNMT1/L1TD1 DKO over DNMT1 KO (log2FC ≥1,

    Techniques Used: Comparison, Mass Spectrometry, RNA Sequencing

    Figure 4. L1TD1 promotes L1 retrotransposition. (A) Schematic representation of plasmids used for retrotransposition (figure modified from Kopera et al., 2016 and generated with BioRender.com). The pJJ101/L1.3 construct contains the full-length human L1.3 element with a blasticidin deaminase gene (mblast) inserted in antisense within the 3’UTR. The mblast gene is disrupted by an intron and mblast expression occurs only when L1 transcript is expressed, reverse transcribed, and inserted into the genome. The pJJ105/L1.3 plasmid contains a mutation in the reverse transcriptase (RT), resulting in defective retrotransposition. The backbone plasmid pCEP4 was used as additional negative control. The blasticidin deaminase gene containing plasmid pLenti6.2 was used as transfection/selection control. (B) Workflow of retrotransposition assay. DNMT1 KO and DNMT1/L1TD1 DKO cells were separately transfected with pJJ101 and control plasmids. Equal number of cells were seeded for each condition. Blasticidin selection (10 µg/ml) was started at day 4 and resistant colonies were counted on day 13. This panel was created using BioRender.com. (C) Bar graph showing the average number of retrotransposition events per 106 cells seeded in three independent experiments. Blasticidin-resistant colonies in pLenti6.2 transfected cells were used for normalization. Statistical significance was determined using unpaired t-test. All data in the figure are shown as a mean of ± SD of three independent experiments, ****p≤0.0001. (D) Representative pictures of bromophenol blue stainings of blasticidin-resistant colonies for each genotype and each transfection.
    Figure Legend Snippet: Figure 4. L1TD1 promotes L1 retrotransposition. (A) Schematic representation of plasmids used for retrotransposition (figure modified from Kopera et al., 2016 and generated with BioRender.com). The pJJ101/L1.3 construct contains the full-length human L1.3 element with a blasticidin deaminase gene (mblast) inserted in antisense within the 3’UTR. The mblast gene is disrupted by an intron and mblast expression occurs only when L1 transcript is expressed, reverse transcribed, and inserted into the genome. The pJJ105/L1.3 plasmid contains a mutation in the reverse transcriptase (RT), resulting in defective retrotransposition. The backbone plasmid pCEP4 was used as additional negative control. The blasticidin deaminase gene containing plasmid pLenti6.2 was used as transfection/selection control. (B) Workflow of retrotransposition assay. DNMT1 KO and DNMT1/L1TD1 DKO cells were separately transfected with pJJ101 and control plasmids. Equal number of cells were seeded for each condition. Blasticidin selection (10 µg/ml) was started at day 4 and resistant colonies were counted on day 13. This panel was created using BioRender.com. (C) Bar graph showing the average number of retrotransposition events per 106 cells seeded in three independent experiments. Blasticidin-resistant colonies in pLenti6.2 transfected cells were used for normalization. Statistical significance was determined using unpaired t-test. All data in the figure are shown as a mean of ± SD of three independent experiments, ****p≤0.0001. (D) Representative pictures of bromophenol blue stainings of blasticidin-resistant colonies for each genotype and each transfection.

    Techniques Used: Modification, Generated, Construct, Expressing, Reverse Transcription, Plasmid Preparation, Mutagenesis, Negative Control, Transfection, Selection, Control



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    reference identifiers additional information antibody dnmt1  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology reference identifiers additional information antibody dnmt1
    Figure 1. DNA hypomethylation results in the activation of L1TD1 expression and loss of L1TD1 affects cell viability in HAP1 cells. (A) Quantification of DNA methylation levels at the L1TD1 promoter in HAP1 wildtype (WT), <t>DNMT1</t> KO, and DNMT1/L1TD1 DKO cells using the MethyLight assay. DNA methylation is shown as percentage of methylation ratio (PMR). (B) qRT-PCR analysis of L1TD1 mRNA expression in HAP1 WT, DNMT1 KO, and DNMT1/ L1TD1 DKO cells. GAPDH was used as a normalization control and relative L1TD1 mRNA levels in DNMT1 KO cells were set to 1. Data are shown as a
    Reference Identifiers Additional Information Antibody Dnmt1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+identifiers+additional+information+antibody+dnmt1/10__7554_slash_elife__96850-283-13-23?v=Santa+Cruz+Biotechnology
    Average 95 stars, based on 1 article reviews
    reference identifiers additional information antibody dnmt1 - by Bioz Stars, 2026-07
    95/100 stars
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    Figure 1. DNA hypomethylation results in the activation of L1TD1 expression and loss of L1TD1 affects cell viability in HAP1 cells. (A) Quantification of DNA methylation levels at the L1TD1 promoter in HAP1 wildtype (WT), DNMT1 KO, and DNMT1/L1TD1 DKO cells using the MethyLight assay. DNA methylation is shown as percentage of methylation ratio (PMR). (B) qRT-PCR analysis of L1TD1 mRNA expression in HAP1 WT, DNMT1 KO, and DNMT1/ L1TD1 DKO cells. GAPDH was used as a normalization control and relative L1TD1 mRNA levels in DNMT1 KO cells were set to 1. Data are shown as a

    Journal: eLife

    Article Title: The domesticated transposon protein L1TD1 associates with its ancestor L1 ORF1p to promote LINE-1 retrotransposition

    doi: 10.7554/elife.96850

    Figure Lengend Snippet: Figure 1. DNA hypomethylation results in the activation of L1TD1 expression and loss of L1TD1 affects cell viability in HAP1 cells. (A) Quantification of DNA methylation levels at the L1TD1 promoter in HAP1 wildtype (WT), DNMT1 KO, and DNMT1/L1TD1 DKO cells using the MethyLight assay. DNA methylation is shown as percentage of methylation ratio (PMR). (B) qRT-PCR analysis of L1TD1 mRNA expression in HAP1 WT, DNMT1 KO, and DNMT1/ L1TD1 DKO cells. GAPDH was used as a normalization control and relative L1TD1 mRNA levels in DNMT1 KO cells were set to 1. Data are shown as a

    Article Snippet: DOI: https://doi.org/10.7554/eLife.96850 13 of 22 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody DNMT1 (H- 300) (polyclonal rabbit) Santa Cruz sc20701 RRID:AB_2293064 1:1000 Antibody ɣ-H2AX (monoclonal mouse) Millipore JBW301 RRID:AB_2847865 1:1000 Antibody Histone H3 C- term (polyclonal rabbit) Abcam Ab1791 RRID:AB_302613 1:5000 Antibody Cleaved caspase 3 (polyclonal rabbit) Cell Signaling 9661 RRID:AB_2341188 1:50 Antibody Anti- mouse HRP (polyclonal) Jackson Laboratories 115-035-008 RRID:AB_2313585 1:10,000 Antibody Anti- rabbit HRP (polyclonal) Jackson Laboratories 211- 032- 171 RRID:AB_2339149 1:10,000 Antibody Goat anti- Rabbit IgG (H+L) Alexa Fluor Plus 488 Invitrogen A32731 RRID:AB_2633280 1:500 Antibody Goat anti- Mouse IgG (H+L) Alexa Fluor Plus 546 Invitrogen A11030 RRID:AB_2737024 1:500 Sequence- based reagent hL1TD1 _f This paper PCR primers CTTA CCCT GG TAGC CGAC CT Sequence- based reagent hL1TD1 _r This paper PCR primers GGCT GGCA AA TTTT CTAA GG Sequence- based reagent hARMC1_f This paper PCR primers AGCT CTGG AG CGAA TTTA AGA Sequence- based reagent hARMC1_r This paper PCR primers GGCA GACA TC CCTG ATCC TG Sequence- based reagent hYY2_f This paper PCR primers TCCC GGAT AG CATT GAAG AC Sequence- based reagent hYY2_r This paper PCR primers TTGA CCTG CA TTTG CTTC TG Sequence- based reagent hORF1p_f This paper PCR primers AGTG CTTA AAG GAGC TGAT GG Sequence- based reagent hORF1p_r This paper PCR primers AACT GGAA GAA AGGG TATC AGC Commercial assay or kit CellTiter- Glo Luminiscent Cell Viability Assay Promega G7571 Commercial assay or kit Monarch RNA Cleanup Kit New England Biolabs T2047L Commercial assay or kit Qubit RNA High Sensitivity kit Thermo Fisher Scientific Q32852 Commercial assay or kit iScript cDNA synthesis Kit Bio- Rad 1708891 Commercial assay or kit Wizard Genomic DNA isolation kit Promega Commercial assay or kit EZ DNA Methylation Kit Zymo Research D5001 Software, algorithm DESeq2 Love et al., 2014 RRID:SCR_015687 Software, algorithm TEtranscript Jin et al., 2015 RRID:SCR_023208 Continued on next page Continued Kavaklioglu et al. eLife 2024;13:RP96850.

    Techniques: Activation Assay, Expressing, DNA Methylation Assay, Methylation, Quantitative RT-PCR, Control

    Figure 3. L1TD1 cross-talk with its ancestor L1 ORF1p. (A) Volcano plot displaying the comparison of the proteomes of HAP1 DNMT1 KO and DNMT1/ L1TD1 DKO cells determined by mass spectrometry. Differentially abundant proteins were plotted as DNMT1/L1TD1 DKO over DNMT1 KO (log2FC ≥1, adj. p-value<0.05 [red] and log2FC ≤ –1, adj. p-value<0.05 [blue]). (B) Volcano plot illustrating the DESeq2 analysis of RNA-seq performed with HAP1 DNMT1 KO and DNMT1/L1TD1 DKO cells. Differentially expressed genes are plotted as DNMT1/L1TD1 DKO over DNMT1 KO (log2FC ≥1,

    Journal: eLife

    Article Title: The domesticated transposon protein L1TD1 associates with its ancestor L1 ORF1p to promote LINE-1 retrotransposition

    doi: 10.7554/elife.96850

    Figure Lengend Snippet: Figure 3. L1TD1 cross-talk with its ancestor L1 ORF1p. (A) Volcano plot displaying the comparison of the proteomes of HAP1 DNMT1 KO and DNMT1/ L1TD1 DKO cells determined by mass spectrometry. Differentially abundant proteins were plotted as DNMT1/L1TD1 DKO over DNMT1 KO (log2FC ≥1, adj. p-value<0.05 [red] and log2FC ≤ –1, adj. p-value<0.05 [blue]). (B) Volcano plot illustrating the DESeq2 analysis of RNA-seq performed with HAP1 DNMT1 KO and DNMT1/L1TD1 DKO cells. Differentially expressed genes are plotted as DNMT1/L1TD1 DKO over DNMT1 KO (log2FC ≥1,

    Article Snippet: DOI: https://doi.org/10.7554/eLife.96850 13 of 22 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody DNMT1 (H- 300) (polyclonal rabbit) Santa Cruz sc20701 RRID:AB_2293064 1:1000 Antibody ɣ-H2AX (monoclonal mouse) Millipore JBW301 RRID:AB_2847865 1:1000 Antibody Histone H3 C- term (polyclonal rabbit) Abcam Ab1791 RRID:AB_302613 1:5000 Antibody Cleaved caspase 3 (polyclonal rabbit) Cell Signaling 9661 RRID:AB_2341188 1:50 Antibody Anti- mouse HRP (polyclonal) Jackson Laboratories 115-035-008 RRID:AB_2313585 1:10,000 Antibody Anti- rabbit HRP (polyclonal) Jackson Laboratories 211- 032- 171 RRID:AB_2339149 1:10,000 Antibody Goat anti- Rabbit IgG (H+L) Alexa Fluor Plus 488 Invitrogen A32731 RRID:AB_2633280 1:500 Antibody Goat anti- Mouse IgG (H+L) Alexa Fluor Plus 546 Invitrogen A11030 RRID:AB_2737024 1:500 Sequence- based reagent hL1TD1 _f This paper PCR primers CTTA CCCT GG TAGC CGAC CT Sequence- based reagent hL1TD1 _r This paper PCR primers GGCT GGCA AA TTTT CTAA GG Sequence- based reagent hARMC1_f This paper PCR primers AGCT CTGG AG CGAA TTTA AGA Sequence- based reagent hARMC1_r This paper PCR primers GGCA GACA TC CCTG ATCC TG Sequence- based reagent hYY2_f This paper PCR primers TCCC GGAT AG CATT GAAG AC Sequence- based reagent hYY2_r This paper PCR primers TTGA CCTG CA TTTG CTTC TG Sequence- based reagent hORF1p_f This paper PCR primers AGTG CTTA AAG GAGC TGAT GG Sequence- based reagent hORF1p_r This paper PCR primers AACT GGAA GAA AGGG TATC AGC Commercial assay or kit CellTiter- Glo Luminiscent Cell Viability Assay Promega G7571 Commercial assay or kit Monarch RNA Cleanup Kit New England Biolabs T2047L Commercial assay or kit Qubit RNA High Sensitivity kit Thermo Fisher Scientific Q32852 Commercial assay or kit iScript cDNA synthesis Kit Bio- Rad 1708891 Commercial assay or kit Wizard Genomic DNA isolation kit Promega Commercial assay or kit EZ DNA Methylation Kit Zymo Research D5001 Software, algorithm DESeq2 Love et al., 2014 RRID:SCR_015687 Software, algorithm TEtranscript Jin et al., 2015 RRID:SCR_023208 Continued on next page Continued Kavaklioglu et al. eLife 2024;13:RP96850.

    Techniques: Comparison, Mass Spectrometry, RNA Sequencing

    Figure 4. L1TD1 promotes L1 retrotransposition. (A) Schematic representation of plasmids used for retrotransposition (figure modified from Kopera et al., 2016 and generated with BioRender.com). The pJJ101/L1.3 construct contains the full-length human L1.3 element with a blasticidin deaminase gene (mblast) inserted in antisense within the 3’UTR. The mblast gene is disrupted by an intron and mblast expression occurs only when L1 transcript is expressed, reverse transcribed, and inserted into the genome. The pJJ105/L1.3 plasmid contains a mutation in the reverse transcriptase (RT), resulting in defective retrotransposition. The backbone plasmid pCEP4 was used as additional negative control. The blasticidin deaminase gene containing plasmid pLenti6.2 was used as transfection/selection control. (B) Workflow of retrotransposition assay. DNMT1 KO and DNMT1/L1TD1 DKO cells were separately transfected with pJJ101 and control plasmids. Equal number of cells were seeded for each condition. Blasticidin selection (10 µg/ml) was started at day 4 and resistant colonies were counted on day 13. This panel was created using BioRender.com. (C) Bar graph showing the average number of retrotransposition events per 106 cells seeded in three independent experiments. Blasticidin-resistant colonies in pLenti6.2 transfected cells were used for normalization. Statistical significance was determined using unpaired t-test. All data in the figure are shown as a mean of ± SD of three independent experiments, ****p≤0.0001. (D) Representative pictures of bromophenol blue stainings of blasticidin-resistant colonies for each genotype and each transfection.

    Journal: eLife

    Article Title: The domesticated transposon protein L1TD1 associates with its ancestor L1 ORF1p to promote LINE-1 retrotransposition

    doi: 10.7554/elife.96850

    Figure Lengend Snippet: Figure 4. L1TD1 promotes L1 retrotransposition. (A) Schematic representation of plasmids used for retrotransposition (figure modified from Kopera et al., 2016 and generated with BioRender.com). The pJJ101/L1.3 construct contains the full-length human L1.3 element with a blasticidin deaminase gene (mblast) inserted in antisense within the 3’UTR. The mblast gene is disrupted by an intron and mblast expression occurs only when L1 transcript is expressed, reverse transcribed, and inserted into the genome. The pJJ105/L1.3 plasmid contains a mutation in the reverse transcriptase (RT), resulting in defective retrotransposition. The backbone plasmid pCEP4 was used as additional negative control. The blasticidin deaminase gene containing plasmid pLenti6.2 was used as transfection/selection control. (B) Workflow of retrotransposition assay. DNMT1 KO and DNMT1/L1TD1 DKO cells were separately transfected with pJJ101 and control plasmids. Equal number of cells were seeded for each condition. Blasticidin selection (10 µg/ml) was started at day 4 and resistant colonies were counted on day 13. This panel was created using BioRender.com. (C) Bar graph showing the average number of retrotransposition events per 106 cells seeded in three independent experiments. Blasticidin-resistant colonies in pLenti6.2 transfected cells were used for normalization. Statistical significance was determined using unpaired t-test. All data in the figure are shown as a mean of ± SD of three independent experiments, ****p≤0.0001. (D) Representative pictures of bromophenol blue stainings of blasticidin-resistant colonies for each genotype and each transfection.

    Article Snippet: DOI: https://doi.org/10.7554/eLife.96850 13 of 22 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody DNMT1 (H- 300) (polyclonal rabbit) Santa Cruz sc20701 RRID:AB_2293064 1:1000 Antibody ɣ-H2AX (monoclonal mouse) Millipore JBW301 RRID:AB_2847865 1:1000 Antibody Histone H3 C- term (polyclonal rabbit) Abcam Ab1791 RRID:AB_302613 1:5000 Antibody Cleaved caspase 3 (polyclonal rabbit) Cell Signaling 9661 RRID:AB_2341188 1:50 Antibody Anti- mouse HRP (polyclonal) Jackson Laboratories 115-035-008 RRID:AB_2313585 1:10,000 Antibody Anti- rabbit HRP (polyclonal) Jackson Laboratories 211- 032- 171 RRID:AB_2339149 1:10,000 Antibody Goat anti- Rabbit IgG (H+L) Alexa Fluor Plus 488 Invitrogen A32731 RRID:AB_2633280 1:500 Antibody Goat anti- Mouse IgG (H+L) Alexa Fluor Plus 546 Invitrogen A11030 RRID:AB_2737024 1:500 Sequence- based reagent hL1TD1 _f This paper PCR primers CTTA CCCT GG TAGC CGAC CT Sequence- based reagent hL1TD1 _r This paper PCR primers GGCT GGCA AA TTTT CTAA GG Sequence- based reagent hARMC1_f This paper PCR primers AGCT CTGG AG CGAA TTTA AGA Sequence- based reagent hARMC1_r This paper PCR primers GGCA GACA TC CCTG ATCC TG Sequence- based reagent hYY2_f This paper PCR primers TCCC GGAT AG CATT GAAG AC Sequence- based reagent hYY2_r This paper PCR primers TTGA CCTG CA TTTG CTTC TG Sequence- based reagent hORF1p_f This paper PCR primers AGTG CTTA AAG GAGC TGAT GG Sequence- based reagent hORF1p_r This paper PCR primers AACT GGAA GAA AGGG TATC AGC Commercial assay or kit CellTiter- Glo Luminiscent Cell Viability Assay Promega G7571 Commercial assay or kit Monarch RNA Cleanup Kit New England Biolabs T2047L Commercial assay or kit Qubit RNA High Sensitivity kit Thermo Fisher Scientific Q32852 Commercial assay or kit iScript cDNA synthesis Kit Bio- Rad 1708891 Commercial assay or kit Wizard Genomic DNA isolation kit Promega Commercial assay or kit EZ DNA Methylation Kit Zymo Research D5001 Software, algorithm DESeq2 Love et al., 2014 RRID:SCR_015687 Software, algorithm TEtranscript Jin et al., 2015 RRID:SCR_023208 Continued on next page Continued Kavaklioglu et al. eLife 2024;13:RP96850.

    Techniques: Modification, Generated, Construct, Expressing, Reverse Transcription, Plasmid Preparation, Mutagenesis, Negative Control, Transfection, Selection, Control